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Image Search Results
Journal: Neuron
Article Title: Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition
doi: 10.1016/j.neuron.2022.04.017
Figure Lengend Snippet:
Article Snippet: The following antibodies were used in this study: rabbit polyclonal anti-β-actin (Abcam; Cat# ab8227; RRID: AB_2305186; LOT# GR3314266-1), mouse monoclonal anti-calbindin (Swant; Cat# 300; RRID: AB_10000347; LOT# 17 (F)), goat polyclonal anti-calretinin (Swant; Cat# CG1; RRID: AB_10000342; LOT# 1§.1), mouse monoclonal anti-CamKII alpha (Thermo Fisher Scientific; 6G9; Cat# Ma1-048; RRID: AB_325403; LOT# TH269517), mouse monoclonal anti-cannabinoid receptor 1 (Immunogene; IMG-3C2; Cat# IMG-CB1R-mAb001; LOT# CJ03), guinea pig polyclonal anti-cholecystokinin (Synaptic Systems, Cat# 438004; RRID: AB_2814938; LOT# 1-1), mouse monoclonal anti-GAD67 (Millipore; 1G10.2; Cat# MAB5406; RRID: AB_2278725; LOT# 3015328), rabbit polyclonal anti-GAPDH (Enogene; Cat# E1C604; LOT# R14Q12), mouse monoclonal anti-gephyrin (Synaptic Systems; mAb7a; Cat# 147021; RRID: AB_2232546; LOT# 147021/15), mouse monoclonal anti-gephyrin (Synaptic Systems; mAb7a; Cat# 147011; RRID: AB_887717; LOT# 147011/54), rat monoclonal anti-HA (Roche; 3F10; Cat# 11867431001; RRID: AB_390919; LOT# 34502100), rabbit monoclonal anti-HA (Cell Signaling; 3724; Cat# 3724; RRID: AB_1549585; LOT# 9),
Techniques: Recombinant, Reporter Assay, Multiplex Assay, Plasmid Preparation, Software, Microscopy
Journal: bioRxiv
Article Title: Cell specificity of adeno-associated virus (AAV) serotypes in human cortical organoids
doi: 10.1101/2023.04.13.536491
Figure Lengend Snippet: ( a & b ) Example of immunohistological sample of Iso clone, 42 days post transduction with AAV6 and labeled with either (a) GFP, MAP2, and DAPI or (b) GFP, GFAP, and DAPI. Dotted square indicates enlarged area. White arrows indicate co-staining of GFP with either (b) MAP2 or (b) GFAP. All hCOs were harvested on day 120, 10 days post AAV transduction. ( c ) Flow cytometry quantification of % GFP labeled cells for each AAV serotype in each hCO clone. ( d ) Flow cytometry quantification of % GFP and MAP2 co-labeled cells for each AAV serotype in each hCO clone. ( e ) Flow cytometry quantification % GFP and GFAP co-labeled cells for each AAV serotype in each hCO clone. All hCOs were harvested on day 120, 10 days post AAV transduction. ( n = 4 hCOs per condition, statistical significance between AAV6 and AAV2 or AAV9 serotypes determined by one-way ANOVA with Tukey post-hoc tests, *** p < 0.001, ** p < 0.01. Values for comparisons between other serotypes are in Supplementary Tables 1-9).
Article Snippet: For staining, slides were rinsed with PBS, then incubated in 0.25% Triton X-100 with 4% Donkey serum (EMD Millipore, S30) for 1 hour, followed by incubation in primary antibodies overnight: chicken GFP (1:3000, Aves Labs, GFP-1010) and
Techniques: Transduction, Labeling, Staining, Flow Cytometry